Table 1.
Primer sequences for quantitative polymerase chain reaction
| Gene name | Primer sequence | Product size | Annealing temperature |
|---|---|---|---|
| ACAN | Forward: 5'-AGGCAGCGTGATCCTTACC-3' Reverse: 5'-GGCCTCTCCAGTCTCATTCTC-3' |
136 bp | 60°C |
| COL2A1 | Forward: 5'-CGTCCAGATGACCTTCCTACG-3' Reverse: 5'-TGAGCAGGGCCTTCTTGAG-3' |
122 bp | 60°C |
| SOX9 | Forward: 5'-TGGGCAAGCTCTGGAGACTTC-3' Reverse: 5'-ATCCGGGTGGTCCTTCTTGTG-3' |
98 bp | 60°C |
| MMP3 | Forward: 5'-TGGCATTCAGTCCCTCTATGG-3' Reverse: 5'-AGGACAAAGCAGGATCACAGTT-3' |
116 bp | 60°C |
| MMP9 | Forward: 5'-GGTGATTGACGACGCCTTTGC-3' Reverse: 5' CGCGACACCAAACTGGATGAC 3' |
115 bp | 60°C |
| MMP13 | Forward: 5'-AAGGAGCATGGCGACTTCT-3' Reverse: 5'-TGGCCCAGGAGGAAAAGC-3' |
72 bp | 60°C |
| GAPDH | Forward: 5'-CGCTCTCTGCTCCTCCTGTT-3' Reverse: 5'-CCATGGTGTCTGAGCGATGT-3' |
82 bp | 60°C |
| B2M | Forward: 5'-GACTTGTCTTTCAGCAAGGA-3' Reverse: 5'-ACAAAGTCACATGGTTCACA-3' |
106 bp | 60°C |