Figure 3.

Average native sequence recovery for CDR H3 loops in germline and mature antibodies. The following design simulations were performed: single-constraint design for the bound conformation (white bar), the free conformation (grey bar) and multi-constraint design for both conformations (black bar) for germline and mature antibodies crystallized in different bound and free conformations (Table I). The star indicates that there is a statistically significant difference (as determined by a Binomial test) between the native sequence recovery obtained from multi- and single-constraint design simulations for germline antibodies.