. Author manuscript; available in PMC: 2014 Apr 8.

Published in final edited form as: Proteins. 2009 Jun;75(4):846–858. doi: 10.1002/prot.22293

Table III.

Dataset of antibodies differing in their degree of maturation after exposure to the same antigen.

Antibody	Antigen	Germline		Mature

		Bound-free	Cα RMSD (Å)	Bound-free	Cα RMSD (Å)
7g12	MMP	1n7m-1ngz	1.0	1ngw-1ngy	0.2
28b4	AAH	1fl6-1fl5	1.8	1kel-1kem	0.2
az-28	HOP	1d6v-1d5i	1.2	1axs-1d5b	0.7
s25-2/s45-18	KDO-trisaccharide¹	1q9q-1q9k	2.2	1q9w-1q9o	0.3
48g7	NPE	1aj7-2rcs	0.8	1gaf-1hkl	0.1
36–65	dodecapeptide	2a6i-2a6j	0.6	N.A.-1jfq	N.A.
d44.1/f10.6.6²	Lysozyme	1mlc-1mlb	0.3	1p2c-2q76	0.1
hyhel-26/hyhel-8²	Lysozyme	1ndm-N.A.	N.A.	1ndg-N.A.	N.A.

The ligand in the mature form contains the KDO-trisaccharide epitope as part of a pentasaccharide

Antibodies isolated after different extents of exposure to the antigen (1mlc,1mlb and 1ndm have a shorter exposure than 1p2c, 2q76 and 1ndg, respectively)

N.A. not applicable

Abbreviations used: MMP, N-methylmesoporphyrin; AAH, 1-[n-4'-nitrobenzylcarboxybutylamino] methylphosphonic acid; HOP, (1s,2s,5s)2-(4-glutaridylbenzyl)-5-phenyl-1-cyclohexanol; NPE, 5-(para-nitrophenyl phosphonate)-pentanoic acid