Figure 2.

Analysis of platelet FIX expression in 2bF9 lentivirus (LV)-transduced recipients. Platelet-FIX expression in 2bF9 LV-transduced primary recipients was monitored for 33 weeks. Isolated platelets were lysed in 0.5% CHAPS. The levels of FIX:Ag in platelet lysates were determined by ELISA assay and FIX:C were determined by chromogenic assay. Platelets from FIX^null mice were used as a negative control. Data shown were summarized from 9 and 7 recipients in the 1100 cGy and the 660 cGy group respectively and expressed as the mean ± SD. (a) Average expression levels of platelet-FIX:Ag at each time point. (b) Average expression levels of platelet-FIX:Ag over the study period. (c) Average expression levels of platelet-FIX:C at each time point. (d) Average expression levels of platelet-FIX:C over the study period. (e) Ratio of platelet-FIX: C to FIX: Ag in 2bF9 LV-transduced mice at each time point. (f) Average ratio of platelet-FIX:C to FIX:Ag over the study period.