Figure 3.

Analysis of FIX expression in the plasma of 2bF9 lentivirus (LV)-transduced recipients. (a) Quantification of the levels of FIX expression in mouse plasma. Plasmas were collected from 2bF9 LV-transduced recipients and hFIX:Ag were quantified by the human FIX-specific ELISA assay. Normal pool of human plasma was used as the standards. Plasmas from FIX^null and wild-type C57BL6 (WT) mice were used as controls. (b) The distribution of the FIX transgene product in mouse whole blood. To analyze the distribution of the FIX between platelets and plasma, we normalized FIX levels to total whole-blood hFIX content in individual mice. 1^° BMT*: data presented in this group were summarized from the primary recipients from which bone marrow mononuclear cells were collected and transplanted into the secondary recipients.