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. 2013 Dec 10;22(1):102–111. doi: 10.1038/mt.2013.240

Figure 1.

Figure 1

The EphA2-TEA-VV express functional EphA2-T-cell engagers. (a) Scheme of expression cassettes encoding EphA2-scFv-CD3-scFv (EphA2-TEA-VV) or GFP (GFP-VV). (b) EphA2-TEA-VV–infected cells secrete bispecific EphA2-scFv-CD3-scFv with the ability of binding to T cells and EphA2. A549 tumor cells (1 × 106/2 ml/well in 6-well plate) were infected with EphA2-TEA-VV or GFP-VV at a multiplicity of infection of 5. The culture media was collected after 24 hours of incubation and added to 1 × 106 unstimulated peripheral blood mononuclear cells (PBMCs), followed by staining with EphA2-FITC, CD8-PE, and CD4-APC. EphA2-FITC, EphA2 protein labeled with fluorescein isothiocyanate; EphA2-TEA-VV, EphA2-T-cell engager-armed vaccinia virus; PE, phycoerythrin; scFv, single-chain variable fragment.