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. 2013 Oct 29;22(1):81–91. doi: 10.1038/mt.2013.216

Figure 1.

Figure 1

In vitro efficacy of SYL040012 and rSYL040012 in human and rabbit cells. (a) Time course inhibition of ADRB2 in human cells: BxPC3 and MDA-MB-231 cells were transfected with either 100 nmol/l SYL040012 or 100 nmol/l of a scrambled sequence siRNA, RNA was isolated and expression of ADRB2 was analyzed 24, 48, and 72 hours after transfection by qPCR. (b) Time course inhibition of ADRB2 in rabbit cells: RAB-9 cells were transfected with either 100 nmol/l of rSYL040012 or 100 nmol/l of a scrambled sequence siRNA, RNA was isolated and expression of ADRB2 was analyzed 24, 48, and 72 hours after transfection by qPCR. (c) Dose-dependent inhibition of ADRB2 in response to SYL040012 in BxPC3 cells 48 hours after transfection. (d) Dose-dependent inhibition of ADRB2 in response to rSYL040012 in RAB-9 cells 48 hours after transfection. (e) Expression of adrenergic receptors β2, β1, and αB1 in human cells (BxPC3 and MDA-MB-231) in response to 100 nmol/l SYL040012, the same dose of a scrambled sequence or phosphate-buffered saline (PBS) (vehicle). (f) Expression of adrenergic receptors β2, β1, and αB1 in rabbit cells (RAB-9) in response to 100 nmol/l rSYL040012, the same dose of a scrambled sequence or PBS (vehicle). Results show means ± SEM of five independents experiments. Statistical significance was calculated by unpaired Student's t-tests and was as follows: *P < 0.05; **P < 0.005; ***P < 0.001 versus time 0 (a–d) or versus vehicle (e–f).