Figure 4.

Proliferation of AAV8 capsid-specific memory CD8⁺ T cells in AAV8-hF.IX-transduced mice. Thy1.1 BALB/c mice were intravenously injected with 2 × 10¹¹ vg of AAV8-hF.IX. Mice then received 5 × 10⁷ CFSE-labeled splenocytes from the Thy1.2 BALB/c mice, which had been immunized with 1 × 10¹¹ vp of Ad-AAV8cap 3–4 months earlier, at day 2, weeks 1, 3, or 7 following AAV8 transfer. The proliferation of Thy1.2⁺CD8⁺AAV8-tet⁺ cells was tested 10 days later from the spleens of the recipient mice. Control mice did not receive AAV8-hF.IX before the adoptive transfer of splenocytes. (a) Average proliferation indices (average number of division cycles) ⁺SD are shown on the left, average % cells proliferating ⁺ SD are shown on the right. (b) Shows representative histograms from a mouse for each time point. Numbers of mice/group were as follows: day 2 = 3 mice, week 1 = 5 mice, week 3 = 3 mice, week 7 = 3 mice, controls (combined from the different time points of this series of experiments) = 12 mice. Significance was determined by multiple Student's t test with Holm–Sidak correction for type 1 errors comparing experimental to control mice. For the proliferation indices, P values were not significant. For % cells proliferating, P values were significant for week 1 (P = 0.01). Significant differences to controls are indicated by (*).