Figure 6.

The stability of ssAAV8(MOVA)-hF.IX vector. Thy1.1 BALB/c mice were injected intravenously with 2 × 10¹¹ vg of AAV8(MOVA)-hF.IX. On day 2, mice received 5 × 10⁷ CFSE-labeled splenocytes from Thy1.2 BALB/c mice, which had been immunized with 1 × 10¹¹ virus particles of the Ad-AAV8cap vector 4 months earlier. Proliferation of Thy1.2⁺CD8⁺AAV8-tet⁺ cells were tested 10 days later using lymphocytes isolated from spleens. The control mice were intravenously injected with phosphate-buffered saline before splenocytes were adoptively transferred. The average number of division cycle (proliferation index) and the percentage of cells of the original sample which divided (% divided) were calculated. Error bars represent SD for two to three mice per group.