Figure 2.

Southern blot analysis and vector copy number in the lentiviral vector-induced liver tumors. (a) Southern blot analysis of genomic DNA extracted from liver tissue of an untransduced control mouse (lane 11), a tumor-free transduced mouse (lane 1), tissue surrounding tumors (lanes 2, 3, and 8), and vector-induced tumors (lanes 4–7 and lanes 9–10). (b) Vector copy number (VCN) per 100 cells was determined by quantitative polymerase chain reaction on the DNA samples. M, male; F, female; T, tumor; S, surrounding nontumorous tissue; TF, tumor-free transduced mouse; control, untransduced mouse.