Table 1.

Primers used in real-time reverse-transcription polymerase chain reaction amplification

Gene	Primer nucleotide sequences
Renin	Forward 5′ AGG ATC AGT GCT GAA TGG GGT GA 3′
	Reverse 5′ GGT TGT GAA TCT CAC AGG CAG TGT 3′
Angiotensinogen	Forward 5′ CAG CAC GAC TTC CTG ACT TGG AT 3′
	Reverse 5′ GGA TGC TGT TGA GAA CCT CTC CCA 3′
Angiotensin II type 1 receptor	Forward 5′ GCT CTG CCA CAT TCC CTG AGT TA 3′
	Reverse 5′ CTT GGG GCA GTC ATC TTG GAT TCT 3′
Brain natriuretic peptide	Forward 5′ AAT CCA CGA TGC AGA AGC TGC T3′
	Reverse 5′ GCG CCG ATC CGG TCT ATC TTC T3′
Angiotensin-converting enzyme	Forward 5′ TGC CTA GAT CCC AAG GTG ACT TTG A 3′
	Reverse 5′ CAA CTT CAT GGC ATC TGC CAG CA 3′
Fibroblast growth factor receptor-1	Forward 5′ ACA GGG GAG GAG GTG GAG 3′
	Reverse5′ACG GTT TGG TTT GGT GTT GT 3′
β2 microglobulin	Forward 5′ ATC TGT CCT TCA GCA AGG ACT GGT 3′
	Reverse 5′ TGG TCC AGA TGA TTC AGA GCT CCA 3′

All primers were designed according to the cDNA sequence of each gene deposited in the GenBank database.