Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Feb 3;16(1):R39. doi: 10.1186/ar4468

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Drouot et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Aspect of anti-HMGCR autoantibodies in indirect immunofluorescence on HEp-2 cells. Classical indirect immunofluorescence assay using the HEp-2000^™ line as cellular substrate. After incubation of serum at a 1/80 dilution, immunoreactivity was revealed by a FITC-labeled anti-human immunoglobulin (Ig)G secondary antibody. Example from two representative anti-HMGCR positive patients out of 20/33 sera with this immunofluorescence pattern shows a finely granular cytoplasmic staining with perinuclear reinforcement (left and middle). Inhibition by free HMGCR protein before immunofluorescence (right): the serum from example 2 was pre-incubated with free HMCGR before immunofluorescence assay. This yielded an extinction of immunoreactivity. HMGCR, 3-hydroxy-3-methylglutaryl coenzyme A reductase.