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. 2013 Aug 15;15(4):R65. doi: 10.1186/bcr3459

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Copyright © 2013 Kaanta et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Flow cytometric analysis of MMTVrtTA/H2BGFP expression within the mammary stem cell and progenitor populations. Total mammary epithelial cell (MEC) population of four-week-old MMTVrtTA/H2BGFP mice, expressing: (a) H2BGFP (b) CD24/CD29 (c) CD24/CD49f. MMTVrtTA/H2BGFP expression within: (e) the CD24⁺/CD29⁺ gate; (f) the CD24⁺/CD29^lo+ gate; (h) the CD24⁺/CD49f⁺ gate; and (i) the CD24⁺/CD49f^lo gate. Stem cell marker expression within the H2BGFP⁺ population: (d) CD24/CD29 (g) CD24/CD49f. H2BGFP, histone 2B-eGFP; MMTV, mouse mammary tumor virus promoter; rtTA, reverse tetracycline transactivator.