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. 2013 Aug 15;15(4):R65. doi: 10.1186/bcr3459

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Copyright © 2013 Kaanta et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vivo outgrowth of H2BGFP⁺/CD24⁺/CD29 ^locells. Outgrowths from H2BGFP⁺/CD24⁺/CD29^lo cells were stained with Carmine Alum for whole-mount analysis, then sectioned and immunostained to analyze their epithelial origin and composition. (a) mammary fat pad whole mount (b) DAPI, GFP and β1 integrin staining (c) CK8, GFP and p63 staining (d) CK14, GFP and β1 integrin staining (e) DAPI, GFP and milk staining. Panel b, 10× magnification. Panels c-e, 60× magnification. Arrowheads indicate representative co-expressing cells. DAPI, 4',6-diamidino-2-phenylindole; GFP, green fluorescent protein; H2BGFP, histone 2B-eGFP.