Table 1.
Tissue donor and sample classification details
|
Donor details |
Sample classification |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Ref. | Source | Age (y) | Level | Intact | cDNA | IL-16 IHC | CCL2 IHC | CCL7 IHC | CXCL8 IHC |
| 1 |
S |
42 |
L4/L5 |
No |
I |
4.0* |
4.0* |
4.2* |
4.0* |
| 2 |
S |
40 |
L5/S1 |
Yes |
I |
7.3* |
7.3* |
5.7* |
7.3* |
| 3 |
S |
25 |
L4/L5 |
Yes |
D |
4 |
4 |
4.7 |
4 |
| 4 |
S |
48 |
L4/L5 |
Yes |
NA |
4.0* |
4.0* |
5.7* |
4.0* |
| 5 |
S |
33 |
L5/S1 |
Yes |
D |
NA |
NA |
NA |
NA |
| 6 |
S |
NA |
NA |
NA |
NA |
6.0* |
6.0* |
7.0* |
6.0* |
| 7 |
S |
62 |
L4/L5 |
Yes |
NA |
7.5 |
7.5 |
7.3 |
7.5 |
| 8 |
S |
32 |
L5/S1 |
Yes |
N |
2.3 |
2.3 |
3 |
2.3 |
| 9 |
S |
26 |
L5/S1 |
Yes |
NA |
NA |
3.3 |
NA |
3.3 |
| 10 |
S |
53 |
L4/L5 |
No |
NA |
7.5* |
7.5* |
7.5* |
7.5* |
| 11 |
S |
40 |
L5/S1 |
Yes |
NA |
7.4 |
7.4 |
5.5 |
7.4 |
| 12 |
S |
66 |
L5/S1 |
NA |
NA |
5.7* |
5.7* |
5.5* |
5.7* |
| 13 |
S |
34 |
L4/L5 |
Yes |
NA |
6.2 |
6.2 |
7.5 |
6.2 |
| 14 |
S |
NA |
NA |
Yes |
D |
NA |
NA |
NA |
NA |
| 15 |
S |
45 |
L5/S1 |
Yes |
D |
NA |
NA |
NA |
NA |
| 16 |
S |
26 |
L4/L5 |
Yes |
I |
NA |
NA |
NA |
NA |
| 17 |
S |
23 |
L4/L5 |
Yes |
N |
NA |
NA |
NA |
NA |
| 18 |
S |
29 |
L4/L5 |
Yes |
I |
NA |
NA |
NA |
NA |
| 19 |
S |
35 |
L5/S1 |
No |
N |
NA |
NA |
NA |
NA |
| 20 |
S |
20 |
L4/L5 |
No |
I |
NA |
NA |
NA |
NA |
| 21 |
S |
39 |
L5/S1 |
No |
I |
NA |
NA |
NA |
NA |
| 22 |
PM |
45 |
L4/L5 |
Yes |
N |
1.8 |
1.8 |
2 |
1.8 |
| 23 |
PM |
45 |
L3/L4 |
Yes |
N |
3 |
3 |
2.5 |
3 |
| 24 |
PM |
45 |
L5/S1 |
Yes |
NA |
3.5 |
3.5 |
3.5 |
3.5 |
| 25 |
S |
48 |
L4/L5 |
No |
D |
9.5 |
9.5 |
6 |
9.5 |
| 26 |
S |
26 |
L5/S1 |
No |
D |
11 |
11 |
3.5 |
11 |
| 27 |
S |
33 |
L5/S1 |
Yes |
I |
8.5* |
8.5* |
6.5* |
8.5* |
| 28 |
PM |
NA |
L1/L2 |
Yes |
NA |
10 |
10 |
6 |
10 |
| 29 |
PM |
NA |
L4/L5 |
Yes |
NA |
8 |
8 |
6 |
8 |
| 30 |
PM |
NA |
L5/S1 |
Yes |
NA |
7 |
7 |
9 |
7 |
| 31 |
PM |
NA |
L3/L4 |
Yes |
NA |
8 |
8 |
7.3 |
8 |
| 32 |
PM |
NA |
L2/L3 |
Yes |
NA |
11 |
11 |
10.3 |
11 |
| 33 |
S |
42 |
L5/S1 |
Yes |
N |
NA |
NA |
NA |
NA |
| 34 |
S |
36 |
L5/S1 |
Yes |
D |
8 |
8 |
4 |
8 |
| 35 |
S |
41 |
L5/S1 |
Yes |
D |
7.5 |
NA |
7.5 |
NA |
| 36 |
S |
NA |
NA |
NA |
I |
6.0* |
6.0* |
4.7* |
6.0* |
| 37 |
S |
44 |
L5/S1 |
Yes |
I |
3.0* |
3.0* |
2.3* |
3.0* |
| 38 |
S |
52 |
L4/L5 |
Yes |
D |
10.5 |
10.5 |
7 |
10.5 |
| 39 |
S |
NA |
L4/L5 |
No |
I |
9.0* |
9.0* |
6.2* |
9.0* |
| 40 |
S |
38 |
L5/S1 |
No |
I |
5.8* |
5.8* |
5.2* |
5.8* |
| 41 |
S |
28 |
L4/L5 |
Yes |
D |
7.7 |
7.7 |
3.5 |
7.7 |
| 42 |
S |
43 |
L5/S1 |
No |
D |
NA |
NA |
NA |
NA |
| 43 |
S |
44 |
L5/S1 |
Yes |
D |
NA |
NA |
NA |
NA |
| 44 |
S |
28 |
L5/S1 |
No |
D |
NA |
NA |
NA |
NA |
| 45 |
S |
35 |
L5/S1 |
Yes |
D |
NA |
NA |
NA |
NA |
| 46 |
S |
43 |
L5/S1 |
No |
D |
NA |
NA |
NA |
NA |
| 47 |
S |
42 |
L5/S1 |
No |
D |
NA |
NA |
NA |
NA |
| 48 |
S |
62 |
L3/L4 |
Yes |
I |
NA |
NA |
NA |
NA |
| 49 |
S |
40 |
L3/L4 |
Yes |
D |
NA |
NA |
NA |
NA |
| 50 | S | 45 | L4/L5 | No | I | NA | NA | NA | NA |
S, surgical; PM, post-mortem; I, infiltrated; D, degenerate; N, non-degenerate, IHC, immunohistochemistry; CCL, C-X-C chemokine. Surgically obtained prolapsed tissue samples were considered to be from intact IVDs when no signs of annulus fibrosus or cartilaginous endplate rupture were evident (protrusion-type prolapse). Values given for IHC sample classification are average histologically determined grades of degeneration; *infiltrating immune cells were observed; NA, not applicable.