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. 2013 Oct 25;33(5):e00076. doi: 10.1042/BSR20130086

Figure 3. Rnf2 knock down enhances α-ENaC mRNA expression and promoter activity in mIMCD3 cells.

Figure 3

(A) mIMCD3 cells transfected with control or Rnf2-specific siRNAs were subjected to qRT-PCR analysis of Rnf2 and GAPDH mRNAs. Rnf2 mRNA was normalized to that of GAPDH. *P<0.05 compared with control, n=3. (B) mIMCD3 cells transfected as in (A) were treated with vehicle (white bars) or 1 μM aldosterone (aldo, black bars) for 24 h. α-ENaC and GAPDH mRNA levels were measured and normalized as in (A). *P<0.05 compared with vehicle, control siRNA; **P<0.05 compared with aldo, control siRNA, n=3. (C) mIMCD3 cells stably expressing an α-ENaC promoter-luciferase construct were transiently transfected and treated with vehicle (white bars) or aldo (black bars) as in (B). After 24 h, cell lysates were prepared for luciferase activity measurements, which were normalized to cell protein content. *P<0.05 compared with vehicle, control siRNA; **P<0.05 compared with aldo, control siRNA, n=3.