. Author manuscript; available in PMC: 2014 Oct 24.

Published in final edited form as: J Phys Chem B. 2013 Jun 25;117(42):12878–12886. doi: 10.1021/jp402104r

Table 2.

Normalized Intensities, Rates, and Relative Amplitudes of Different Phases for Changes in Dns Fluorescence during Cyt c Unfolding Induced by Interactions with CL-containing Liposomes^a,^b

Variant	Relative Intensities			Phase 1		Phase 2^c	Phase 3
Variant	I_burst/I_U (%)	/I_U (%)	/I_U (%)	k₁ (s^-1)	a_{1, vscl}^d (%)	a_{2, vscl}^d (%)	k₃×10³ (s^-1)	a_{3, vscl}^d (%)
Dns28	12.5	23.2	34.5	0.43 ± 0.27	21.4	48.5	3.3 ± 0.1	30.1
Dns39	12.0	21.0	28.3	0.61 ± 0.09	31.0	40.2	4.4 ± 1.5	28.8
Dns50	9.8	22.0	51.3	0.52 ± 0.02	23.8	17.7	0.9 ± 0.1	58.5
Dns66	4.1	14.5	47.4	0.30 ± 0.01	11.8	16.0	0.9 ± 0.1	72.2
Dns92	1.4	9.7	44.6	0.35 ± 0.01	7.3	9.7	1.2 ± 0.1	83.0
Dns99	3.5	19.3	31.5	0.71 ± 0.03	16.0	37.1	5.7 ± 0.9	46.9
Dns104	2.7	10.3	40.7	0.90 ± 0.05	3.1	7.1	3.2 ± 0.3	89.8

TOCL/DOPC liposomes (vscl, 50 mol% CL).

Determined with a combination of stopped-flow (Phases 1 and 2) and manual (Phase 3) mixing experiments in a 25 mM HEPES pH 7.4 at final concentrations of 3 μM of Dns-labeled cyt c and 750 μM of total lipid. All measurements were performed at 22±2 °C.

Kinetics progress curves for all the variants during Phase 2 could be fit to the process with the rate constant k₂ having values between 0.03 and 0.04 s^-1.

Percent of the observable amplitude for the Dns fuorescence signal during 60 min after mixing (a_{1, vscl} + a_{2, vscl} + a_{3, vscl}=100%, the burst phase is not included).