FIGURE 1.
Generation of a stable C. elegans line expressing P23H bovine opsin. A, two-dimensional topology of the bovine opsin sequence (black letters) compared with human (green) and mouse (blue). Mutations in adRP cases are shown with a black background and those in arRP cases are shown with gray. The P23H mutation is featured with red background as indicated by a black arrow. B, shown are the P23H opsin and DsRed expression constructs injected into C. elegans for expression of bovine P23H opsin and DsRed in neurons. Both constructs contain a neuronal driving PH20 promoter (blue block) followed by the codon-optimized cDNA of bovine opsin with a Pro → His mutation at codon 23 (green block) or the cDNA of DsRed (red block).