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. 2014 Feb 10;289(13):9288–9303. doi: 10.1074/jbc.M114.551713

FIGURE 6.

FIGURE 6.

Mass spectrometry-based disulfide bond analysis of P23H isorhodopsin. A, tandem mass spectrum of P23H isorhodopsin peptide VFGPTGCNLEGFF with the precursor ion of 722.83(2+) (m/zcharge). The series b or y ions from b5 to b12 and y2 to y8 clearly show CAM labeling at Cys-110. B, tandem mass spectrum of P23H isorhodopsin peptide IPEGMQCSCGIDYY with the precursor ion of 888.85(2+). The series b ions from b5 to b13 clearly show NEM labeling at Cys-185 and CAM labeling at Cys-187. Met-193 is oxidized.