TABLE 1.
Mass spectrometry of chymotrypsin-digested P23H isorhodopsin and WT isorhodopsin purified from C. elegans for disulfide bond analysis
Proteins were purified and characterized as described under “Experimental Procedures.”
| Peptide sequence | Location | Cys in peptide | WT label |
P23H label |
Note | ||
|---|---|---|---|---|---|---|---|
| NEM | CAM | NEM | CAM | ||||
| VFGPTGCNL | 104–112 | Cys-110 | − | + | − | + | CAM |
| VFGPTGCNLEGFF | 104–116 | Cys-110 | − | + | − | + | CAM |
| GPTGCNL | 106–112 | Cys-110 | − | + | − | + | CAM |
| GPTGCNLEGFF | 106–116 | Cys-110 | − | + | − | + | CAM |
| GPTGCNLEGFFATL | 106–119 | Cys-110 | − | + | − | + | CAM |
| SRYIPEGMQCSCGIDYY | 176–192 | Cys-185, Cys-187 | − | + | − | + | 2 CAM |
| SRYIPEGMQCSCGIDYY | Cys-185, Cys-187 | + | + | + | + | 1NEM and 1CAM | |
| IPEGMQCSCGIDY | 179–191 | Cys-185, Cys-187 | − | + | − | + | 2 CAM |
| IPEGMQCSCGIDY | Cys-185, Cys-187 | + | + | + | + | 1NEM and 1CAM | |
| IPEGMQCSCGIDYY | 179–192 | Cys-185, Cys-187 | − | + | − | + | 2 CAM |
| IPEGMQCSCGIDYY | Cys-185, Cys-187 | + | + | + | + | 1NEM and 1CAM | |
| QCSCGIDYY | 184–192 | Cys-185, Cys-187 | − | + | − | + | 2 CAM |