FIGURE 5.

Silencing RAGE reduced the cystic area originating from each nephron segment in PC2R mice. A, kidney sections stained with tubule-specific markers. Kidney sections were stained with tubule-specific antibodies to classify cyst origin. The antibodies used were anti-DBA (green) for collecting duct staining, anti-LTA (green) for the proximal tubule, and anti-THP antibody (green) for the distal tubule. The nuclei were stained with DAPI (blue). Representative data are presented (magnification, ×100). Scale bars, 200 μm. B, statistical analysis of cystic area in each case. The IMA module of MetaMorph software was utilized to calculate cystic area (each >40 cysts). Data are mean ± S.D. (error bars). *, p < 0.001.