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. 2014 Feb 19;289(13):9247–9253. doi: 10.1074/jbc.M114.551143

FIGURE 2.

FIGURE 2.

CDK12 depletion causes HR defects in ovarian cancer cell lines. A–C, OVCAR-8 cells were transfected twice with control luciferase (Luc), BRCA1-1, CDK12-1, or CDK12-2 siRNAs. 48 h after transfection, the cells were analyzed for CDK12, BRCA1, and RAD51 expression (A), analyzed for DNA content (B), or irradiated with 10 grays of ionizing radiation and stained to detect RAD51 foci (C). Mean ± S.D., n = 4; p values by paired t test. D, OVCAR-8-DR-GFP cells transfected with pCβASceI plasmid plus control luciferase (Luc), BRCA1, CDK12-1, or CDK12-2 siRNAs were analyzed by flow cytometry for GFP fluorescence 72 h after transfection. Data in D were normalized to luciferase-transfected cells for each experiment. Mean ± S.D., n = 4; p values by paired t test.