FIGURE 5.

HIV-2 Vpx does not inhibit IRF3 and IRF7 transactivation activity. 293T cells in a 12-well plate were transfected with pRL-TK control plasmid (100 ng), IFNβ-luc reporter plasmid (300 ng), and IRF3 or IRF7 expression plasmid (1 μg) together with an increasing amounts of Vpx expression plasmid (0, 1, and 3 μg) as indicated. Immunoblot analysis of whole cell lysates prepared for luciferase assay is shown below. In all transfections, the pCNF vector was added to bring the total plasmid to the same amount. Luciferase activity was measured at 24 h post-transfection by Dual-Luciferase reporter assay. Relative luciferase activity was determined as fold induction (relative to the basal level of reporter genes in the presence of pCNF vector after normalization with co-transfected relative luciferase units). Values are mean ± S.D. for three experiments.