FIGURE 2.

A, growth phenotypes of WT and TbGT11 mutant cells. Cells were inoculated into culture, counted, and diluted to 1 × 10⁴ cells/ml with fresh media every 2 days. The growth characteristics for WT (black triangles), TbGT11 conditional null cells under permissive (plus tetracycline, gray diamonds) and non-permissive (minus tetracycline, gray squares) conditions were indistinguishable. B, infectivity of WT and TbGT11 null mutant bloodstream-form parasites to mice. Mice were infected with 1 × 10⁵ cells of WT (black) or TbGT11 null mutants (gray) and parasite cell numbers were determined after 1, 2, and 3 days. No difference in infectivity was observed between WT and TbGT11 null mutant cells.