FIGURE 5.

Lectin blotting of total glycoproteins. Western blot of total glycoproteins (depleted for VSG) of WT (lanes 1, 4, and 7), TbGT11 conditional null mutants grown under permissive conditions (lanes 2, 5, and 8) and TbGT11 conditional null mutants grown under non-permissive conditions (lanes 3, 6, and 9) incubated with ricin (left-hand panel). Binding specificity was confirmed by preincubating the lectin with 10 mg/ml of galactose and lactose (middle panel). Ponceau S staining shows equal loading and transfer between the lanes (right-hand panel).