FIGURE 7.
Serpinb6b protects target cells from mouse, but not human, granzyme A-induced death. A, survival curve of P815 cells transfected with pEF-puro (open squares) or pEF-puro and pEF-IRES-GFP/Serpinb6b (closed squares) exposed to a sublytic concentration of streptolysin-O and a serial dilution series of mGzmA for 1 h at 37 °C, with survival measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay 18 h later. EC50 values were derived by fitting a sigmoidal dose-response equation by nonlinear regression. B, bar graph summarizing all experiments indicating means ± S.D. **, p < 0.01.