TABLE 1.

Kinetic parameters for granzyme A cleavage of peptide substrates

Data shown are mean ± S.D. of three independent experiments. Substrates included an N-terminal aminobenzoyl fluorophore and a C-terminal dinitrophenyl lysine quencher and aspartic acid to enhance solubility.

	Mouse Granzyme A		Human Granzyme A
	GLFRSLSS	VANRSAS	GLFRSLSS	VANRSAS
kcat (s−1)	33.5 ± 2.94	14.2 ± 2.13	2.51 ± 0.0642 × 10−2	4.88 ± 0.233
Km (μm)	114 ± 15.4	38.3 ± 6.62	53.5 ± 0.382	35.2 ± 3.46
kcat/Km (m−1 s−1)	2.95 ± 0.476 × 105	3.70 ± 0.848 × 105	4.69 ± 0.124 × 102	1.39 ± 0.152 × 105