FIGURE 1.

RTT109 is required for qiRNA and quelling pathway. A, QDE-2 Western blot analysis of a panel of Neurospora knock-out strains, including rtt109 (NCU09825), from the unbiased screen. All of the strains were grown in histidine (1 mg/ml) for 2 days. B and C, Northern blot analysis of qiRNA and aRNA products from the wild-type strain and rtt109 mutant. Cultures were treated with 1 mg/ml histidine for 2 days. Ethidium bromide (EB)-stained membranes were used as loading controls. D, quelling efficiency of the indicated strains. The numbers in the parentheses indicate the number of strains examined after the quelling assays.