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. 2014 Feb 19;289(13):9365–9371. doi: 10.1074/jbc.M113.528521

FIGURE 3.

FIGURE 3.

VPS75 and ASF1 are required for qiRNA production. A, silver-stained SDS-PAGE gel showing the affinity purified Myc-RTT109 products. Cell extracts of the rtt109KO and rtt109KO strain expressing Myc-RTT109 were used for purification. B, Western blot showing the levels of H3K56Ac in the indicated strains. Cultures were grown with/without 1 mg/ml HU for 2 days. C and D, Northern blot analysis of aRNA and qiRNA production in the indicated strains. EB, ethidium bromide.