FIGURE 3.
VPS75 and ASF1 are required for qiRNA production. A, silver-stained SDS-PAGE gel showing the affinity purified Myc-RTT109 products. Cell extracts of the rtt109KO and rtt109KO strain expressing Myc-RTT109 were used for purification. B, Western blot showing the levels of H3K56Ac in the indicated strains. Cultures were grown with/without 1 mg/ml HU for 2 days. C and D, Northern blot analysis of aRNA and qiRNA production in the indicated strains. EB, ethidium bromide.