FIGURE 5.

H3K56Ac and Myc-RAD51 is enriched around DSB. A, Western blot analysis showing the level of H3K56Ac in the rtt109 and wild-type strains grown in indicated concentration of HU. B, Western blot analysis showing the level of γΗ2Α in the indicated strains. γΗ2Α was examined after cultures were treated with 0.1 μg/ml CPT treatment for 2 days. C, ChIP-quantitative PCR analysis showing the enrichment of MycRAD51 around the I-SceI-induced DSB, which is flanked by the al-1 sequence. A primer set detecting the am-1 gene is used as a negative control D, ChIP assays showing the H3K56Ac enrichment around the DSB following the same procedure in C (n = 3). Error bars indicate S.D.