FIGURE 10.

ST6GAL-I overexpression counteracts NEU1-mediated inhibition of EC capillary-like tube formation. A, HPMECs, HPMECs infected with Ad-GFP (MOI = 100), and HPMECs infected with increasing MOIs of Ad-ST6GAL-I were seeded at 1.5 × 10⁴ ECs/well and incubated for 6 h on Matrigel, at which time they were photographed, and branches of capillary-like tubes were counted. B, photomicrographs of HPMECs infected with Ad-GFP (i) or Ad-NEU1 (ii), each at MOI = 100, or co-infected with both Ad-NEU1 and Ad-ST6GAL-I, each at MOI = 100, after 6 h incubation on Matrigel. Magnification is ×10. C, HPMECs and HPMECs infected with Ad-GFP or Ad-NEU1, each at MOI = 100, or co-infected with Ad-NEU1 (MOI = 100) and increasing MOIs of Ad-ST6GAL-I were incubated for 6 h on Matrigel and photographed, and branches of capillary-like tubes were counted. In A and C, each vertical bar represents mean ± S.E. (error bars) branches/HPF. The MOI and n for each condition are indicated below each bar. *, significantly decreased compared with the simultaneous Ad-GFP-infected control at p < 0.05.