FIGURE 5.

TDG regulates colon cancer cell growth in vitro and in vivo. A—C, TDG is required for the proliferation of colon cancer cells. Stable colon cell lines that expressed control shRNA (ctrlshRNA) and TDG shRNAs were established. Top panels, cell proliferation assays. *, p < 0.05. Bottom panels, TDG expression levels in the stable cell lines. D, deletion TDG inhibited colon cancer cell growth in vivo. Stable HT29 cell lines that expressed control shRNA and TDG shRNAs were inject subcutaneously into nude mice. Growth curves of these xenografted tumors were analyzed. *, p < 0.05; **, p < 0.01; ***, p < 0.001. E, xenografted tumors from control and TDG-depleted cells.