FIGURE 3.

Lgr4 inactivation increases cell apoptosis in multiple organs. A and B, apoptosis was dramatically increased in Lgr4^−/− kidney. Representative 4-week-old wild type and Lgr4^−/− kidney samples are shown. C and D, increased apoptosis was found in Lgr4^−/− testis. Representative 4-week-old wild type and Lgr4^−/− testis samples are shown. E and F, significantly increased apoptosis was present in Lgr4^−/− ovary corpus luteum. Representative 4-week-old wild type and Lgr4^−/− ovary samples are shown. G and H, increased apoptosis appeared in the Lgr4^−/− uterus. Representative 4-week-old wild type and Lgr4^−/− uterus samples are shown. Cell proliferation was decreased in spermatogonia and Leydig cells in Lgr4^−/− testis. Scale bar = 10 μm. ST, seminiferous tubule; GC, granulosa cell; CL, corpus luteum; L, stroma layer.