| Reagent | Final concentration |
|---|---|
| KCl | 150 mm |
| Sucrose | 250 mm |
| Tris–HCl | 10 mm |
| β-Mercaptoethanol | 1.4 mm |
Adjust the pH of the solution to 7.3 with 1 m KOH before adding β-mercaptoethanol. Prepare the solution freshly every 3–4 d. Store at 4°C.
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