Table 2.
A set of gene fragments recommended as an international standard for multilocus sequence typing of C. albicans.
| Locus | Gene product | Chromosome | Primer sequence (5′→3′) | Amplicon sizes (bp) | |
|---|---|---|---|---|---|
| AAT1a | Asparate aminotransferase | 2 | Fwd | ACTCAAGCTAGATTTTTGGC | 478 |
| Rev | CAGCAACATGATTAGCCC | ||||
| ACC1 | Acetyl-coenzyme A carboxylase | 3 | Fwd | GCAAGAGAAATTTTAATTCAATG | 519 |
| Rev | TTCATCAACATCATCCAAGTG | ||||
| ADP1 | ATP-dependent permease | 1 | Fwd | GAGCCAAGTATGAATGATTTG | 537 |
| Rev | TTGATCAACAAACCCGATAAT | ||||
| MPIb | Mannose phosphate isomerase | 2 | Fwd | ACCAGAAATGGCCATTGC | 486 |
| Rev | GCAGCCATGCATTCAATTAT | ||||
| SYA1 | Alanyl-RNA synthetase | 6 | Fwd | AGAAGAATTGTTGCTGTTACTG | 543 |
| Rev | GTTACCTTTACCACCAGCTTT | ||||
| VPS13 | Vacuolar protein sorting protein | 4 | Fwd | TCGTTGAGAGATAATCGACTT | 741 |
| Rev | ACGGATGGATCTCCAGTCC | ||||
| ZWF1b | Glucose-6-phosphate dehydrogenase | 1 | Fwd | GTTTCATTTGATCCTGAAGC | 702 |
| Rev | GCCATTGATAAGTACCTGGAT |