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. Author manuscript; available in PMC: 2014 Apr 8.
Published in final edited form as: Arthritis Rheum. 2010 Jul;62(7):1993–2003. doi: 10.1002/art.27473

Figure 3.

Figure 3

Involvement of transcriptional regulation in the expression of cytokines and chemokines to resistin. A, Resistin stimulates the activity of pNF-κB Luc reporter in TC28 human chondrocytes. Relative luciferase activity indicates the fold expression relative to the activity of zero time (set as 1) in the presence of resistin (100 ng/ml). B, The candidate C/EBPβ and NF-κB binding sites in human CCL3 (-1395) and CCL4 (-1281) constructs. C/EBPβ and IKK2 stimulate the expression of CCL3 (C) and CCL4 (D) promoter in TC28 human chondrocytes. The CCL3 and CCL4 promoter constructs were co-transfected with C/EBPβ and IKK2 expression plasmids into T/C-28a2 cells without or with resistin (100 ng/ml) 8 h (CCL4) or 24 h (CCL3). The luciferase activity of the empty vector in the absence of resistin is set as 1. Each bar represents the mean ± S.D.