Total cell lysates were collected from MUC13 over-expressing cells (SW480 M13OE) (A), MUC13 knock-down cells (SW620 M13KD) (B) and from corresponding vector controls SW480 Vector and SW620 Vector cells, respectively. The lysates were immunoblotted for multiple proteins, including: TERT, p53, SHH, BMI-1, GATA1, Bcl-xl, HER2, ERK and P-ERK. β-actin was used as the internal loading control for all immunoblot analysis. A representative blot from at least 2 independent lysates is shown.