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. Author manuscript; available in PMC: 2015 Mar 25.
Published in final edited form as: Oncogene. 2013 Oct 7;33(39):4746–4755. doi: 10.1038/onc.2013.415

Figure 3.

Figure 3

EPSTI1 promotes the cell invasiveness downstream of KLF8. (a and b) EPSTI1 knockdown decreases the invasiveness of MDA-MB-231 cells. The cells were transfected with siRNA for 72 h. A fraction of the cells was prepared for western blotting (a). Another fraction of the cells was used for Matrigel invasion for 16 h (b) as described in the Material and Methods. Data was normalized to the untreated cells. *P < 0.05. (c) EPSTI1 knockdown inhibits KLF8-promoted invasiveness. The 10A-iK8 cells were cultured under uninduced (U) or induced (I) conditions for 48 h and then treated with or without the indicated siRNA for 72 h. Matrigel invasion was done similarly. Data was normalized to the uninduced, untreated cells. (d-g) EPSTI1 overexpression is sufficient to promote invasiveness. The 10A-iE1 and MCF7-iE1 cells were generated as described in the Material and Methods. After grown under U or I conditions for 24 h, the cells were prepared for western blotting (d, f) and invasion assay (e, g). The invasion data were normalized to the U and serum-free conditions. *P < 0.05. All the data are representatives of at least three independent experiments in duplicate.