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. 2014 Apr 7;55(4):2130–2139. doi: 10.1167/iovs.13-13468

Figure 1.

Figure 1

PKC inhibitors enhance IR-induced reduction in cell viability, cell proliferation, and clonogenic survival of GNAQmt UM cells. (A) The GNAQmt (Mel202, 92.1) and GNAQwt (OCM3) cells were treated with DMSO, BIM (1 μM), or AEB071 (0.5 μM) for 3 hours, followed by 0, 2, 4, or 6 Gy of IR. Cell viability was determined 120 hours after IR by using trypan blue dye. (B) Cells were treated with DMSO, BIM (1 μM), or AEB071 (0.5 μM) for 3 hours followed by 0 or 6 Gy of IR. Cell proliferation was determined 72 hours and 120 hours after IR by using trypan blue dye. (C) Cells were treated with DMSO, BIM (1 μM), or AEB071 (0.5 μM) for 3 hours followed by 0, 2, 4, or 6 Gy of IR. Twenty-four hours after IR, the medium was changed and cells were incubated at 37°C for another 14 days to allow colony formation. The cultures were stained and colonies containing more than 50 cells were counted. Bars represent mean ± SD from three biological replicates; **P < 0.001 for comparing IR only to PKC inhibitor combined with IR at 6 Gy.