Figure 1.

A, representative whole-cell recordings of glial ESCs generated by paired pulse stimulation (10 ms interval) of parallel fibres in transverse cerebellar slices at 0.033 Hz (first panel). Raising stimulation frequency to 0.2 Hz (second panel) and 1 Hz (third panel) for 10 min leads to a progressive depression of ESCs. After returning baseline frequency to 0.033 Hz for 10 min (fourth panel), the persistent depression is evident in comparison with initial recordings (grey trace). B, representative recordings of EPSCs from Purkinje neurons under the same stimulation conditions as in A. Note persistent decrease in decay time. C, aggregate data of EPSC amplitude (Amp) and decay time (Dec) from Purkinje neurons (n = 17) and ESC amplitude in Bergmann glia (BGC; n = 5). Data are mean ± SEM normalized to initial values at 0.033 Hz. *P < 0.0001 (single sample t test). D, plot of change in decay time after recovery against initial ESPC amplitude for all Purkinje neurons (n = 17) with linear regression (dashed line). E, subtraction of ESCs (left panel) and EPSCs (right panel) recorded after recovery from initial recordings at 0.033 Hz shows the current sensitive to depression. Grey traces are from all recorded cells, black traces are mean currents.