Figure 2.

A, first panel: representative EPSCs recorded from Purkinje neurons before (grey trace) and after (black trace) addition of 200 μm TBOA for 10 min, during stimulation at 0.033 Hz. Second panel: raising baseline frequency to 0.2 Hz for 10 min after TBOA treatment decreased both ESPC amplitude and decay time (black trace). Third panel: mean ± SEM aggregate data from n = 7 cells for amplitude (Amp) and decay time (Dec) at 0.033 Hz (grey columns) and 0.2 Hz (black columns) after addition of TBOA, normalized to values before addition of TBOA. *P = 0.009 and 0.001 (paired t test) for change after 0.2 Hz stimulation in mean amplitude and decay time, respectively. B, representative EPSCs recorded from Purkinje neurons before (grey trace) and after (black trace) bath incubation with 2.5% dextran for 5 min. Second panel shows traces normalized to amplitude. Third panel shows mean ± SEM amplitude (Amp) and decay time (Dec) for six cells, normalized to values before addition of dextran. *P = 0.043 and 0.023 (single sample t test) for change in mean amplitude and decay time after dextran, respectively. C, representative EPSCs recorded from Purkinje neurons in slices incubated at 34°C stimulated at 0.033 Hz (grey trace) and 0.2 Hz for 10 min (black trace). Second panel shows traces normalized to amplitude. Third panel shows mean ± SEM amplitude (Amp) and decay time (Dec) for eight cells at 0.2 Hz, normalized to values at 0.033 Hz. *P = 0.034 (single sample t test).