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A, B, cells were stimulated with 70 mm K+ solution for 1 min (red lines below traces) causing a reversible increase in Fluo-4 fluorescence in single HAP1+/+ (A) and HAP1−/− (B) cells. C–E, the average area under the curve (C), peak fluorescence change (D) and time to peak fluorescence (E) were calculated for each genotype (n = 13 cells in each genotype; **P < 0.01).
