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. 2014 Apr 8;9(4):e93394. doi: 10.1371/journal.pone.0093394

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© 2014 Tseng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Ubiquitylation of Fih-1, (B) Trabid C (295–716 aa) and (C) Trabid FL (full-length) by Mib or Mib2. COS7 cells were co-transfected with pcDNA3.1-HA-ubiquitin, pCS2-FLAG tag and pCS2-MT tag constructs. The cell lysate was incubated with anti-Myc antibody and then subjected to IB with anti-HA (top panel) or anti-Myc antibody (bottom panel).