Table 1. Zebrafish Mib/Mib2-binding proteins isolated in the first yeast two-hybrid screen.
| Prey Groups | Zebrafish Prey genes (GenBank accession number) | Human Homolog | Baits | PBS* | Preys Binding region (frequency) |
| Ubiquitin-Proteasome System | proteasome 26S subunit, non-ATPase, 1, (psmd1) (NM_201184) | PSMD1 | I | D | 114 - 320aa (2) |
| ubiquitin specific peptidase 13 (isopeptidase T-3) (usp13) (NM_001098386) | USP13 | II | C | 438 - 889aa (3) | |
| ubiquitin B (ubb) (NM_001013272) | UBB | II | A | full length (31) | |
| mind bomb (mib) (NM_173286) | MIB1 | II | D | 507 - 876aa (1) | |
| ubiquitin A-52 residue ribosomal protein fusion product 1(uba52) (NM_001037113) | UBA52 | II | A | full length (30) | |
| ubiquitin specific protease 5 (usp5) (NM_214755) | USP5 | II | A | 489 - stop codon (31) | |
| ubiquitin specific protease 9 (usp9) (NM_001077449) | USP9 | II | B | 1980 - 2363aa (3) | |
| ubiquitin C (ubc) (NM_001077804) | UBC | II | A | full length (45) | |
| ribosomal protein S27a (rps27a)/UBIQUITIN A-80-RESIDUE RIBOSOMAL PROTEIN FUSION PRODUCT (UBA80) (NM_200502) | RSP27A (UBA80) | II | A | full length (46) | |
| Cytoskeleton | actinin alpha 2 (actn2) (NM_001037573) | ACTN2 | I | D | 528 - stop codon (1) |
| actinin alpha 4 (actn4) (NM_199586) | ACTN4 | I | A | 660 - stop codon (7) | |
| septin 7a (sept7a) (NM_201161) | SEPT7 | II | C | 14 - 198aa (4) | |
| Trafficking | SEC6-like 1; exocyst complex component 3 (exoc3) (NM_212715) | EXOC3 (SEC6) | I | B | full length (4) |
| PREDICTED: Danio rerio similar to EPS15 protein (eps15) (XM_002663099) | EPS15 | II | D | 971 - stop codon (1) | |
| PREDICTED: Danio rerio epsin-2-like, transcript variant 1(epn2) (XM_681373) | EPN2 | II | D | 84 - 265aa (1) | |
| Replication/Transcription/Translation | paired box gene 3 (pax3) (NM_131277) | PAX3 (HUP2) | I | D | 192 - stop codon (2) |
| paired box gene 7 (pax7a) (NM_131332) | PAX7 (HUP1) | I | D | 112 - 397aa (1) | |
| paired box gene 7 (pax7c) (XM_689806) | PAX7 (HUP1) | I, II | D, D | 141- stop codon (3) | |
| orthodenticle homolog 2 (otx2) (NM_131251) | OTX2 | II | D | 19 - 233aa (1) | |
| DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (ddx5) (NM_212612) | DDX5 | I | A | 47 - 273aa (6) | |
| PREDICTED: Danio rerio helicase-like transcription factor-like (hltf) (XM_687979) | HLTF (HIP116) | II | D | 363 - 562aa (1) | |
| Cell Signaling | TGF-beta activated kinase 1/MAP3K7 binding protein 3 (TAK1-binding protein 3) (tab3) (NM_001045105) | TAB3 | II | 338 - 611aa (1) | |
| polo-like kinase 4 (plk4) (NM_001118892) | PLK4 | I | D | 591 - 828aa (1) | |
| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (ywhaz) (NM_212757)(14-3-3 zeta) | YWHAZ (14-3-3 zeta) | II | A | 63 - 299aa (15) | |
| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b (ywhabb), mRNA (14-3-3 beta) (NM_213145) | YWHAB (14-3-3 beta) | II | D | 76 - 263aa (1) | |
| GTP binding protein 4 (gtpbp4) (NM_199851) | GTPBP4 (CRFG) | II | D | 43 - 502aa (1) | |
| Others | 4-aminobutyrate aminotransferase, gamma-aminobutyrate transaminase (abat) (NM_201498) | ABAT (GABAT) | I | D | 156 - stop codon (1) |
| plasma membrane calcium ATPase 4 (atp2b4) (EU559285) | ATP2B4 (PMCA4) | I | B | 626 - 816aa (3) | |
| ankyrin repeat domain 52a (ankrd52a) (NM_001018154) | ANKRD52 | II | D | 230 - 510aa (1) | |
| insulin-degrading enzyme (ide) (NM_001089525) | IDE | II | B | 252 - 813aa (7) | |
| family with sequence similarity 76, member B (fam76b) (NM_199932) | FAM76B | I | B | 1 - 118aa (3) | |
| calcium binding protein 39-like (cab39l) (NM_001007327) | CAB39L | II | D | 11 - 347aa (1) | |
| rapunzel (rpz) (NM_001145238) | unknown | II | D | 47 - unknown (1) | |
| PREDICTED: ankyrin repeat domain-containing protein 13A-like (ankrd13a) (NM_001126387) | ANKRD13A | II | D | 273 - stop codon (1) | |
| PREDICTED: Danio rerio similar to limb expression 1(lix1) (XM_681174) | LIX1 | II | D | 1 - 267aa (1) | |
| PREDICTED: Danio rerio zinc finger protein 569-like (LOC567317) (XM_690612) | unknown | II | D | 11 - 235aa (1) | |
| PREDICTED: Danio rerio similar to Zinc finger protein 521 (LOC571623) (XM_695227) | unknown | II | D | 96 - 221aa (1) | |
| similar to zinc finger like protein (LOC100330312) (XR_117615) | unknown | I | D | unknown (1) |
Notes. Baits I: Mib-C-RF123a (783–1029 amino acid); II: Mib2-C-RF12 (781–999 amino acid).
*PBS (Predicted Biological Score): A = very high confidence in the interaction, B = high confidence in the interaction, C = good confidence in the interaction, D = moderate confidence in the interaction. The association of Mib/Mib2 and two of the preys (Usp5 and Usp9) were chosen for further investigation by co-immunoprecipitation (Co-IP). Preys that have or do not have physical interaction with Mib/Mib2 in Co-IP assay are in bold and italic fonts, respectively.