Table 2. Zebrafish Mib-binding proteins isolated in the second yeast two-hybrid screen.
| Prey Groups | Zebrafish Prey genes (GenBank accession number) | Human Homolog | Baits | Preys Binding region (frequency) |
| Ubiquitin Proteasome System | ubiquitin specific protease 1 (usp1) (NM_199579) | USP1 (UBP) | V | 349 - stop codon (1) |
| proteasome subunit, alpha type, 5 (psma5) (NM_205708) | PSMA5 | V | from start codon (1) | |
| proteasome 26s subunit, non-ATPase, 4 (psmd4) (NM_001002112) | PSMD4 (Rpn10) | III, V | from 80aa (3) | |
| tumor-necrosis-factor-receptor-associated factor-binding domain (trabid)/zinc finger, RAN-binding domain containing 1 (zranb1) (NM_001077768) | ZRANB1 (TRABID) | III | from 295 - stop codon (1) | |
| Cytoskeleton | myosin light chain, phosphorylatable, fast skeletal muscle a (mylpfa) (NM_131188) | MYLPF | III, V | full length (21) |
| atrial myosin light chain (AF434191) | unknown | III | full length (1) | |
| actinin alpha 3 (actn3) (NM_131522) | ACTN3 | V | 680- stop codon (1) | |
| cofilin 1 (non-muscle) (cfl1) (NM_213639) | CFL1 | V | 9 - stop codon (3) | |
| type I cytokeratin, enveloping layer, like (cyt1l) (NM_001082882) | unknown | III, V | from 157aa (3) | |
| Trafficking | exocyst complex component 1 (exoc1) (NM_199597) | EXOC1 (SEC3) | III | from 305aa (1) |
| vesicle docking protein p115 (vdp) (NM_200155) | USO1 (VDP) | III | from 600aa (1) | |
| translocase of outer mitochondrial membrane 20 homolog b (yeast) (tomm20b) (NM_001002698) | TOMM20 (MOM19) | III, V | from15aa (2) | |
| Replication/Transcription/Translation | Danio rerio c20orf14 homolog (H. sapiens), mRNA (c20orf14) (BC066556) | PRPF6 | V | from 430aa (2) |
| cleavage and polyadenylation specific factor 3 (cpsf3) (BC085402) | CPSF3 | III | from start codon (2) | |
| replication protein A (rpa) (NM_131711) | RPA2 (HSSB) | V | from start codon (3) | |
| splicing factor 3a, subunit 3 (sf3a3) (BC096781) | SF3A3 (PRP9) | V | from 302aa (2) | |
| poly A binding protein, cytoplasmic 1 a (pabpc1a) (NM_001031676) | PABPC1 (PABP) | V | from 449aa (1) | |
| v-maf musculoaponeurotic fibrosarcoma (avian) oncogene homolog (maf) (BC065941) | MAF | III, V | from 136aa (3) | |
| atonal homolog-1 (zath-1) (AF024536) | ATOH1 (ATH1) | V | from 38aa (1) | |
| ribosomal protein L27 (rpl27) (NM_199724) | RPL27 | III | from start codon (1) | |
| ribosomal protein, large, P0 (rplp0) (NM_131580) | RPLP0 | III | from 157aa (2) | |
| ribosomal protein L17 homolog (rpl17) (NM_212760) | RPL17 | IV | full length (1) | |
| Cell Signaling | hypoxia-inducible factor 1, alpha subunit inhibitor (hif1an)/factor inhibiting HIF 1 (fih-1) (NM_201496) | HIF1AN (FIH1) | III | full length (1) |
| tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a (ywhaqa) (14-3-3 theta) (BC066409) | YWHAQ (14-3-3 theta) | III | 57 - stop codon (1) | |
| low density lipoprotein receptor-related protein associated protein 1 (lrpap1) (NM_201306) | LRPAP1 | V | from 43aa (1) | |
| B-cell receptor-associated protein 31 (bcap31) (NM_200092) | BCAP31 (BAP31) | III | from start codon (1) | |
| poly ADP-ribose polymerase 12-like, (LOC564886) (XM_688209) | unknown | IV | 938 - stop codon (3) | |
| progesterone receptor membrane component 1 (pgrmc1) (NM_001007392) | PGRMC1 (MPR) | III | full length (1) | |
| creatine kinase, muscle (ckm) (NM_130932) | CKM (CKMM) | III | from 55aa (1) | |
| brain-subtype creatine kinase (ckbb)(AY055849) | CKB (BCK) | III | from 169aa (1) | |
| casein kinase 2 beta (ck2b) (NM_131187) | CSNK2B | V | full length (1) | |
| Others | YY1 associated factor 2 (yaf2) (NM_001045268) | YAF2 | III | 116 - stop codon (1) |
| apolipoprotein A-I (apoa) (NM_131128) | APOA1 | IV | from start codon (1) | |
| apolipoprotein Eb (apoeb) (BC065592) | APOE | III | from start codon (1) | |
| nascent polypeptide-associated complex alpha polypeptide (naca) (BC122432) | NACA | III, V | from 101aa (7) | |
| immunoglobulin binding protein (AY648749) | HSPA5 | III, V | 483 - stop codon (4) | |
| glyceraldehyde-3-phosphate dehydrogenase, transcript variant 1 (gapdh) (XM_679205) | GAPDH | III | from 59aa (1) | |
| GDP dissociation inhibitor 2 (gdi2) (AY391428) | GDI2 | III, V | from 279aa (3) | |
| heat shock cognate (hsc70) (L77146) | HSPA8 (HSC70) | III | 420 - stop codon (2) | |
| heat shock 70 kDa protein 5 (glucose-regulated protein) (hspa5) (BC063946) | HSPA5 (GRP78) | III, V | from 478aa (4) | |
| heat shock protein 90 kDa alpha (cytosolic), class B member 1 (hsp90ab1) (NM_131310) | HSP90AB1 (HSP84) | III | from start codon (1) | |
| heat shock protein 9 (hspa9) (NM_201326) | HSPA9 | III | from 513aa (1) | |
| PREDICTED: Danio rerio wu:fc5af04, transcript variant 1(wu:fc51f04) (XM_680524) | unknown | IV | 431 - stop codon (1) |
Notes. Baits III: Mib-M-Ank (454–785 amino acid); IV: Mib-C-RF123b (789–1030 amino acid); V: Mib-ZnF+Ank (74–785 amino acid). The association of Mib and five of the preys (Usp1, Trabid, Zath-1, Fih-1 and Hsc70) were chosen for further investigation by co-immunoprecipitation (Co-IP). Preys that have and do not have physical interaction with Mib in Co-IP assay are in bold and italic fonts, respectively.