Figure 3. Effect of MNK shRNA.
A) ANBL-6 MM cells infected with lentiviral shRNA targeting MNK1 or MNK2 or a scrambled sequence (SCR, control), followed by immunoblot. B) ANBL-6 cell lines knocked down for MNK1 (1KO) or MNK2 (2KO), seeded at 3×105 cells/ml and then treated +/− IL-6 for 72 hrs with determination of viable cell recovery by trypan blue exclusion. Data shown are mean+/−SD, (n = 5) and * denotes statistically significant reduced recovery in MNK-silenced lines compared to control (C).