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. 2014 Apr 8;9(4):e93936. doi: 10.1371/journal.pone.0093936

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© 2014 SANDES et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Overlay flow cytometric histograms of the control and the MBHA3-treated cells labeled with Rho 123 (FL1-H). The dose-dependent reduction of Rho 123 fluorescence intensity, mainly at the 4x IC₅₀ of MBHA3, indicates the depolarization of the mitochondrial membrane. Histograms representative of duplicate experiments are shown.