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. 2014 Apr 8;9(4):e94122. doi: 10.1371/journal.pone.0094122

Figure 1. Discovery and characterization of disulfide–rich Tv1 teretoxin.

Figure 1

An integrated approach for characterizing sample limited disulfide peptidic natural products was applied: a) RP-HPLC on-line separation of Terebra variegata venom (highlighted is the region where the sequenced peptide eluted). b) ETD and CAD MS/MS analysis recorded on the native peptide to determine the sequence of Tv1 peptide. c) RP-HPLC of linear and folded versions of chemically synthesized Tv1 peptide. d) MS/MS spectrum of partially reduced and alkylated Tv1 used to determine the disulfide connectivity. e) NMR solution structure of folded Tv1 peptide. f) Bioactivity of 20 μM of Tv1 (−) versus normal saline (NS) (−) solution injected into polychaete worms. Y-axis indicates level of activity, where 3 is the normal activity of the NS injected worm, 1 and 2 indicate decreased activity due to partial paralysis.