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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Arthritis Rheum. 2013 Oct;65(10):2703–2712. doi: 10.1002/art.38059

Figure 2.

Figure 2

Induction of Rgs13 in GC B cells by IL-17. A, qRT-PCR analysis of Rgs13 expression in B cells sorted from the spleens of indicated strains (ND = not detectable; ** p<0.01 for the indicated comparisons). B, qRT-PCR analysis of Rgs13 after normalization to Gapdh, in purified BXD2 B cells 2 hrs after treatment with IL-17 or IL-21 (50 ng/ml) and Bcl6, after normalization to Gapdh, in purified BXD2 B cells 2 hrs after treatment with IL-21 (50 ng/ml)(Rt bar). Fold changes of the indicated genes relative to the untreated control (indicated by a gray line) are shown. C, qRT-PCR analysis of basal levels of Il17ra, after normalization to Gapdh, in A20 and 70Z/3 cells. (D) Gel electrophoresis (upper) and qRT-PCR analysis (lower) of Rgs13, after normalization to Gapdh, by A20 and 70Z/3 cells. Cells were stimulation with IL-17 at the indicated time points. Results are shown as the mean ± SEM (ND = not detectable; *** p< 0.005 between the indicated comparison or between basal versus stimulated cells)(N=4 for all panels).